Cane mosaic virus Yunnan isolate TAS-ELISA test kit and its preparing method

This invention discloses a kin of sugar-cane mosaic virus yunnan outlier three antibody sandwich enzyme immune adsorbing rule detection kit. It includes case, enzyme standard board and liquid set in the case. The liquid comprises positive and negative contrast, enzyme standard antibody, buffer solution and substrate. It also includes multiple and single antibodies of sugar-cane mosaic virus yunnan outlier. The single and multiple antibodies take sugar-cane mosaic virus extracting solution with 35-45 mg/ml virus concentration as immune antigen. They are prepared by immune injection method and single clone antibody method. The detection sensitivity of the detection kit in this invention can reach 0.0051-0.01ng/ml, and it is 100-1000 times than indirect ELISA and DAS-ELISA detection sensitivity.