Resonance light scattering of quercetin

Resonance light scattering(RLS) and absorption spectra of Quercetin were studied in the present paper.In the solution with pH 3.30 to pH 6.50,the RLS singal was very strong and stable,but decreased sharply with a increase in pH when pH6.50.In the B-R buffer solution at pH 4.00,the RLS intensity at 497 nm was maximum and proportional to the concentration of quercetin.The linear range is 0.0-3.0×10~(-4) mol·L~(-1) with the detection limit of 3.1×10~(-7) mol·L~(-1).Quantum chemistry calculation showed that this enhancement of RLS occurred because the neutral molecules of quercetin assembled into supermolecular aggregates by 4-4'hydrogen-bond.This result completely accords with the spectral data from experiments.