STUDY ON THE CHANGES OF LEARNING AND MEMORY AND MONOAMINE NEUROTRANSMITTER CONTENTS OF BRAIN IN FOCAL CEREBRAL ISCHEMIA REPERFUSION MICE

Objective:To observe the changes of learning and memory and monoamine neurotransmitter contents of brain in focal cerebral ischemia reperfusion mice.Methods: 24 Kunming mice were used and divided into 3 groups at random: sham-operation group,ischemia 3h reperfusion 18h group and ischemia 3h reperfusion 24h group,each group had 8 mice.Suture method was used to establish ischemia reperfusion mouse model.The learning and memory ability of mice were detected with Morris water maze and the monoamine neurotransmitter contents in brain were detected with HPLC-ECD.Results: The learning and memory ability,the monoamine neurotransmitter contents of brain in cerebral ischemia reperfusion mice were obviously lower than control mice(P0.05,P0.01).Conclusions: Suture method ischemia reperfusion model can decrease the learning and memory ability,the monoamine neurotransmitter contents of brain in mouse.