The canavanine-pentacyanoamine ferrate complex formation.

The complexation of pentacyanoamine ferrate with canavanine is not a specific one. Other amino acids, especially tryptophan and histidine may also react with proper analytical features. Se also do the indolyl and imidazolyl moieties of nonamino acids. Photoactivation does neither seem to occur by exposure of the pentacyanoamine ferrate reagent to daylight, nor is it a perequisite for a more sensitive response of canavanine complexation. On the other hand, oxidants and reducers may well interfere with the absorbance of the complex. In addition molar absorbance, stoichiometric ratio of the complex, and reation-order kinetics were determined. By mean of temperature programming equilibrion constant andreaction kinetics, enthalpy, Gibbs nenergy, entropy values, and activation energy of complex formation were stablished.