Formation of .DELTA.-Tocopherol-cyclodextrin Complexes by the Action of Cyclodextrin Glucanotransferase

Formation of δ-tocopherol (δ-Toc) and cyclodextrin (CDs) complexes were studied by two kinds of methods. The first was a method (the CD wrap method) newly developed by the author, in which the mixture of starch and δ-Toc was reacted with cyclodextrin glucanotransferase (CGTase) in a 20% ethanol solution. The other was an inclusion method, in which the mixture of δ-Toc and CDs was vigorously stirred to form complexes in a water solution. The products prepared by the two methods were analyzed by fast atom bombardment (FAB) mass spectrometry. The peaks corresponding to δ-Toc-α-CD and δ-Toc-β-CD complexes were found in mass spectra of samples prepared by the CD wrap method, whereas such peaks were not found in the mass spectra of the inclusion method samples.