Construction of a cDNA library of the Fungi(E-20)producing aflatoxin-detoxifizyme

Object: To construct a cDNA library of Fungi(E 20) that produce aflatoxin detoxifizyme for screening the genes of the fungi(E 2O). Methods: The cDNA library of Fungi(E 20) were constructed using the SMARTTM cDNA library construction kit. After having constructed the cDNA library, the titer and the recombination rate of the unamplified library were detected and then amplified. Then, ten clones were selected randomly and sequenced. Results: The titer and the recombination rate of the unamplified library were about 1.0×10 6 pfu/ml and 98.9%, and the titer and the capacity of the amplified library were about 3×10 8 pfu/ml and 4×10 10 .Ten expressed sequence taqs (ESTs) were gained from ten clones being sequenced. Blasting in the GenBank, one sample shows high homology with the data of NADH ubiquinone oxidoreductase (NuoC) gene. Accomplishment of this paper is an initial key work for building the resource information databank of the fungus(E 20).