The Status Of Genetic Polymorphism Of CCR2 And SDF1 Genes In Selected Populations Of Madhya Pradesh

Which attack and destroy the Acquired Immune systems of Human called The Acquired ImmuneDeficiency Syndrome (AIDS) disease caused by The Human Immunodeficiency Virus 1 (HIV1).There areseveral genetic factors which play significant role during pathogenesis of HIV1 in Human body. Inpresent study, We have studied the CCR2 and SDF1 genes polymorphism. The total of 105 samples ofcaste population and 92 samples of Korku tribe were analyzed for the earlier established polymorphismsof CCR2 and SDF1 genes, which are associate the delaying the HIV1 replication in Human T cells. Thesamples were collected on The FTA cards. The discs from the cards were separate out using puncturemachine and further processed as per given protocol from the GE Healthcare Life Sciences. In presentinvestigation, the established primers were used for the PCR amplification of the desired fragment ofChemokine Receptor 2 and Stromal derived factor 1 (SDF1). The Amplified PCR products were subjectedto digested with respective restriction enzymes, These RFLP results were further validated by sangersequencing. In the genotype study we could not reported any homozygous mutant in CCR2 gene but it incase of SDF1 gene two individual reported, which carry both the mutant alleles in caste population. Theresults showed the less minor allele frequency of CCR2 and SDF1 all two studied populations. Hence, thestudy concluded that the high risk of HIV1 progression after infection, Due to less protective allele werefound in the studied population of MP.