Exonuclease-assisted target recycling amplification for label-free chemiluminescence assay and molecular logic operations

A versatile exonuclease III (Exo III)-assisted target recycling amplification (Exo-TRA) strategy is demonstrated to achieve label-free and homogeneous chemiluminescence (CL) detection of target DNA. Inspired by this principle, a series of logic gates are constructed using DNA molecules as “inputs” to activate the Exo-TRA system.