Methodology Assays for the Salivary Biomarkers’ Identification and Measurement

In the last decades, the development of assays for the identification and quantification of biomarkers of health and disease has been immense. The increase of sensitivity of those assays allowed their use in saliva samples, although it may require several adaptations. This chapter aims to offer a schematic view of the most important methods to discover and measure salivary biomarkers in human and veterinary species. For academic purposes, the different methodologies have been classified into spectrophotometric assays, immunoassays, and “omics”; however, several techniques may belong to different categories. Finally, examples of the applications of these techniques in saliva are given.