Effects of Extracts from Prinsepia utilis Royle on Antimicrobial Activity

Objective: Under three conditions of water bath at 60 ℃ for 12 h,boiling 10 min and 20 min,water-soluble extract was collected respectively from different parts,including seeds,roots,stems and leaves from Prinsepia utilis Royle.Antimicrobial effects of extracts on eight pathogenic microorganisms were studied.Method: The discs of filter paper were used for antimicrobial determination.Results: Under the above three conditions,extract from seeds and roots exhibited varied antimicrobial activity when their concentration reached 0.25 g/ml.Extract from seeds,under all conditions,showed higher antimicrobial activity than that from roots(p0.05).At the concentration of 0.25 g/ml,extracts by water bath at 60 ℃ for 12 h and by boiling water for 20 min showed greater antimicrobial effects than that by boiling water for 10 min(p0.05);and extending the extraction time could effectively increase the antimicrobial effect.The minimal inhibitory concentration of seeds extract was 0.125 g/ml.Conclusion: Water extracts from seeds and roots of Prinsepia utilis Royle had the antimicrobial effect on the six pathogenic microorganisms.