The construction of prokaryotic expression vector of PEDV membrane protein

To obtain its stable expression,we took two strategies to eliminate the antibacterial phenomenon of membrane protein′ in this work.First,the truncated fragment of PEDV M gene which encodes the exterior of the viral envelope(M′) was subcloned into pGEX-6p-1 for the construction of pGEX-6p-M′.Second,in the same way the recombinant plasmids pGEX-6p-M-GST were constructed with GST genes linking at N-terminal and C-terminal of M gene.