Expression and existence forms of mast cell activating molecules and their antibodies in systemic lupus erythematosus.

Introduction Mast cells are regarded as a kind of classical anaphylaxis cells. However autoimmune diseases and allergic reactions have many similarities or overlaps. A large number of papers have proved that mast cells play a significant role in the pathogenesis of systemic lupus erythematosus (SLE). It is speculated that IgE, anti-IgE antibodies, FceRI, and anti-FceRI antibodies activate mast cells through autoimmune pathways and participate in the disease process of SLE. Naturally occurring protein molecules not only exist in monomer form, but also in polymer of protein molecules. Therefore, whether IgE, FceRIα, anti-IgE antibodies, and anti-FceRI antibodies also exist in polymeric forms in the natural state is worthy of further investigation. Methods The serum samples and clinical data of 131 patients with SLE were collected from Qilu Hospital (Qingdao). Sixty healthy individuals were collected as the control group. Serum FceRIα, anti-IgE, and anti-FceRI were detected by enzyme-linked immunosorbent assay. Serum IgE was detected by rate scatter nephelometry. A Chinese hamster ovarian cancer cell line CHO3D10 transfected with human FceRIα was cultured and the cell protein extract was prepared. The existence forms of FceRIα in the cell protein extract were detected by the native-page method. Results The serum FceRIα in SLE patients was significantly higher than that in control group (3.52 [2.18, 4.71] µg/ml and 1.87 [1.52, 2.33] µg/ml, respectively; p Conclusion In patients with SLE, the expression of FceRIα was increased and the level of anti-IgE was decreased. FceRIα had one kind of monomer and two kinds of polymers. Mast cell-associated FceRIα involved in the inflammatory lesion of SLE.