Identification of sugar apple cultivars Nua and Nang using AFLP and SCAR markers

Amplified fragment length polymorphism (AFLP) technique was used to identify sugar apple ( Annona squamosa L.) cultivars Nua and Nang. The total of 64 Eco RI- Mse I primer-pair combinations were screened and all of them could produce DNA fingerprinting. Eight AFLP markers were clearly polymorphic among the 2 cultivars which were capable to distinguish the sugar apple varieties between Nua and Nang. Four polymorphic AFLP fragments were subsequently cloned and sequenced for sequence characterized amplified region (SCAR) primer design. The primers were tested with individual DNA samples and the result showed the polymorphisms that were identified from the SCAR markers with specific to only sugar apple variety Nua.