The Effects of Caffeic Acid Phenethyl Ester (CAPE), Royal Jelly, and Curcumin on Gene Expression of ADAMTS-1, -5, and -9 in OUMS-27 Chondrosarcoma Cells: A Preliminary Study -

Objective: ADAMTS (A Distintegrin and Metalloproteinase with Thrombospondin motif) genes participate in a number of physiological and pathological functions. Caffeic acid phenethyl ester (CAPE), royal jelly (RJ) and curcumin have been used for the treatment of several diseases as alternative medicine remedies. There are considerable data that these compounds can be used to decrease the symptoms of osteoarthritis (OA). The molecular bases of the effects of these compounds in OA were aimed to be illuminated. Methods: The gene expression analysis for ADAMTS-1, ADAMTS-5, and ADAMTS-9 were carried out by using Real-time PCR method in OUMS-27 chondrosarcoma cells. Results: These enzyme genes that have aggrecanase activity were found to be induced upon IL-1α application. In terms of ADAMTS/β-ACTIN ratio, ADAMTS-1 gene expression was increased by two-fold after IL-1α induction. ADAMTS-5 gene expression was found to be increased by 1.6, 1.3, and 2.7 times, respectively following IL-1α induction. ADAMTS-9 gene expression was found to be elevated by 2.5, 2.9, and 4.2 times, respectively. The compounds that have nuclear factor-kappa B (NF-κB) inhibition potential prevented the elevation of gene expression in different ratios. Conclusion: The findings that these molecules can inhibit the NF-κB cascade which is very important in OA pathogenesis were interpreted as mechanism of action of alternative remedial agents.