Analysis of Multiple Apoptosis Parameters Using a Microfluidic Chip-Based System

We have developed miniaturized assays for detection of phosphatidylserine exposure, caspase activity and DNA laddering using lab-on-a-chip technology. A readout system capable of two-color fluorescence detection was used to run disposable glass chips with various analytical functionalities. The chips contain an interconnected network of microchannels that permits either electrophoretic separations of biomolecules or simple flow cytometric analysis of cells.