Use low ozone dosages to have a positive effect in the cooling and cryopreservation of equine semen

Abstract The aim of this study was to find an ozone dosage capable of inducing pro-oxidation, and to verify its action on sperm cells during the process of cooling and cryopreservation of equine semen. This study evaluates ozone concentrations of 2µg/mL,15µg/mL, 30µg/mL e 60 µg/mL added in equine semen cooling extender and equine semen freezing extender. Samples were evaluated for sperm kinetics patterns, function of sperm structures and lipid peroxidation. In the experiment, the concentration of 15 µg/mL showed higher total and progressive motility when comparing to control (60.3 ± 3 and 40.7 ± 3.4 vs. 54.9 ± 4 e 35.0 ± 4.4, respectively, P