Nitratireductor shengliensis sp. nov., Isolated from an Oil-Polluted

Two Gram-negative, non-motile, short-rod- shaped bacterial isolates, designated 110399 T and 110248, were isolated from an oil-polluted saline soil in Shengli Oilfield, Eastern China. The two strains shared 99.9 % 16S rRNA gene sequence similarity with the DNA-DNA relatedness value being 80.0 %. They were both capable to grow at 20-40 C, pH 7-9, and 1-9 % (w/v) NaCl with the optimum growth happened at 30 C, pH 8, and 2-6 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains were members of Nitratireductor and most closely related to Nitratire- ductor pacificus pht-3B T and N. basaltis J3 T with the 16S rRNA gene sequence similarities being 97.1 and 97.0 %. The DNA-DNA relatedness between the novel strains and two type strains were below 27 ± 7 %. The strains 110399 T and 110248 also differed from N. pacificus and N. basaltis in nitrate reduction, salt tolerance, enzyme activities, and utilization of carbon sources. The major cellular fatty acids of strain 110399 T were C19:0x8c cyclo (10.5 %) and Summed Feature 8 (C18:1x7c and/or C18:1x6c, 41.5 %) which are typical in the genus Nitra- tireductor. The predominant ubiquinone was Q-10. The genome DNA G?C content of strain 110399 T and 110248 was 61.1 and 61.7 mol%. On the basis of genetic, pheno- typic, and chemotaxonomic analyses, strains 110399 T and 110248 represent a novel species within the genus Nitra- tireductor, for which the name Nitratireductor shengliensis sp. nov. is proposed. The type strain is 110399 T (=CGMCC 1.12519 T = LMG 27405 T ).