Behavior of Paramecium sp., treated with bifenazate with special emphasis on respiratory metabolism, protein and generation time

3 Abstract: The human species has never been as vulnerable to its environment, particularly in relation to the disturbances that it even introduced. The problem is essentially that of the urban environment soon where 90% of the population lives, while the sources of toxic exposure to electromagnetic fields continue to multiply. Currently in toxicology, the use of alternative models permits to understand the mechanisms of toxic action at different levels of the organization of the cell. The main objective of our work is to study the effect of the acaricide: the Bifenazate on ciliate protists freshwater: Paramecium sp. To do this, we tested the effect of this xenobiotic at different concentrations (10, 20, 40 and 80µM) on aliquots of 50ml of culture of paramecium done beforehand. The results obtained show that the growth of paramecium is a sensitive to the product and at the high concentrations. The toxicity was evaluated by determining the IC 50 and by calculating the percentage response, which evaluates the response of protists towards the pollutant and thus confirms the evolution of the growth curve. The variation in the rate of total protein concentrations according to Bifenazate compared to the control reflects the ability of microorganisms to the metabolisation/ detoxification. The measurement of respiratory activity shows inhibition of oxygen consumption reflecting a fatal effect of this acaricide.