Abstract 13102: Microrna-150 Modulates Ischemia-induced Neovascularization in Atherosclerotic Conditions

Background: Hypercholesterolemia is an atherosclerotic condition that is associated with impaired neovascularization in response to ischemia. This study sought to define the role of microRNAs (miRNAs) in that pathophysiology. Methods and results: Next generation sequencing and qRT-PCR analyses identified miR-150 as a pro-angiogenic miRNA which expression is significantly reduced in the ischemic muscles of hypercholesterolemic ApoE-deficient (ApoE-/-) mice, and in HUVECs exposed to oxLDL. Forced expression of miR-150 using a miR mimic could rescue oxLDL-mediated impairment of endothelial cell migration and tubule formation in vitro. In a mouse model of hindlimb ischemia, intramuscular injection of miR-150 mimic restored blood flow recuperation, vascular densities in ischemic muscles, and functional mobility in ApoE-/- mice. Treatment of ApoE-/- mice with miR-150 also increased the number and the activities of pro-angiogenic cells (PACs). miR-150 targets SRC kinase signaling inhibitor 1 (SRCIN1), an importa...