Seed Culture of Aromatic Rice Varieties Under Salt Stress

Dehusked seeds of five aromatic rice varieties were placed on MS medium supplemented with NaCl and Na 2 SO 4 salts at four different levels (0%, 0.2%, 0.4% and 0.6%) to observe the callus induction and plant regeneration responses. For callus induction MS medium was supplemented with 2 mg/L 2, 4 D while for plant regeneration MS medium was supplemented with 1 mg/L NAA and BAP. All the genotypes presented significant variation in callus induction and plant regeneration responses. Callus induction and plant regeneration percentage decreased with the increase of salt doses. Among the tested varieties, in most of the cases, Kalijira performed the best both for callus induction and plant regeneration at different levels of salts (0 - 0.6%). It was concluded that salt tolerant aromatic rice varieties can be evolved applying salt stress in in vitro condition.