The biodistribution of melanomacrophages and reactivity of PEG or amine-functionalized iron oxide nanoclusters in the liver and spleen of Egyptian toad after intraperitoneal or oral injections: Histochemical study

Abstract Recently, toad flesh is the main source of protein for many peoples. Of note, disease treatment of amphibian animals is a big challenge facing toad farms development. Iron oxide nanoclusters (IONCs) are approved by the Food and Drug Administration (FDA) as new materials for drug delivery systems development. The biodistribution and fate of IONCs in the lower vertebrate tissues such as toads is novel and should be studied in details. In this study, the biodistribution and toxicities of polyethylene glycol-functionalized IONCs (PEG-IONCs) and amine-functionalized IONCs (NH2-IONCs) in the liver and spleen of Egyptian toad were studied after intraperitoneal or oral injections. The localization and levels of IONCs in liver and spleen depends on the root of injection and the surface functionalization. The presence of IONCs in the liver and spleen produced sever to mild histological and histochemical abnormalities, but in a different ratio. The change of melanomacrophages (MMs) numbers depends on the root of injection or the function group on the surface of IONCs and this explains the abnormalities of MMs produced by IONCs treatment. Further, the function group on the surface may control the biodistribution of MMs and abnormalities produced by IONCs in the liver and spleen. Understanding the biodistribution and histological abnormalities of IONCs in the lower vertebrate tissues (amphibians in this study) might introduce important information to develop new drugs which can be used for amphibian diseases treatment or diagnosis. Further, the histopathological and MMs abnormalities produced by IONCs may consider as biomarkers for amphibians diseases diagnosis.