EGFR/AKT pathways alterations in gliomas

2061 Background: EGFR/AKT pathways anomalies are well known in gliomas. Nevertheless, specific alterations for the different types of gliomas have not been assessed. Our goal was to look for differences in the status of EGFR, 10q and CDKN2A/ARF genes in three groups of gliomas. Methods: We performed molecular analysis of 43 oligodendrogliomas (OD), 22 oligoastrocytomas (OA) and 18 glioblastomas (GBM). LOH at 1p, 19q and 10q was assessed by Real Time PCR quantitative microsatellite analysis. Markers for 10q analysis (D10S536 and D10S1683) are located proximal to PTEN and DMBT-1 loci respectively. EGFR and CDKN2A/ARF were studied by multiplex real time PCR. Clinical information related to diagnosis, tumor characteristics and outcome was also documented. Results: As expected by current knowledge, alterations in EGFR/AKT pathways were highly common in GBM, showing significant differences in EGFR amplification and also 10q and p16 deletion, when compared to OD. In a more detailed analysis, including number of ...