Role of the kidneys in the mechanism of erythorodieresis in hemolytic (phenylhydrazine) and acute posthemorrhagic anemia

The degree of hemolysis was investigated in intact and nephrectomized rats after administration of phenylhydrazine. Hemolytic (phenylhydrazine) anemia in nephrectomized animals is manifested by a smaller decrease in the total erythrocyte count, the percentage of51Cr-labeled erythrocytes, and the intensity of the reaction for hemosiderin in tissues of the reticuloendothelial system. A lower degree of erythrodieresis was found in nephrectomized rats and after acute unreplaced blood loss. Perfusion of blood through the kidney of anemized rats leads to an increase in the plasma potassium concentration of the perfusion fluid, a decrease in the electrophoretic mobility and hemolysis time of the erythrocytes, an increase in their fragility, and a decrease in the proportion of protein fractions with molecular weights of between 74,500 and 27,000 in the erythrocyte stroma.