Delivery system resisting hepatitis B virus and biological preparation

The invention discloses lipid nanoparticles composed of fat polypeptide molecules, dioleoyl phosphatidyl ethanolamine, cholesterol and polyethylene glycol, which coat the sgRNA molecules of Cas9mRNA and target HBV gene conserved domain. The invention also discloses a preparation method and application of the lipid nanoparticles and a pharmaceutical composition and preparation comprising the lipid nanoparticles. The lipid nanoparticles and biological preparation disclosed by the invention can serve as a novel delivery system resisting hepatitis B virus.