Character of interaction between irradiated and non-irradiated cells in culture in diffusion chambers in vivo.

: Meta: vyznachyty harakter vzajemodii' mizh neoprominenymy klitynamy kistkovogo mozku myshej ta oprominenym mikrootochennjam u kul'turi dyfuzijnyh kamer in vivo. Materialy i metody. Dlja vyvchennja osoblyvostej vzajemodii' mizh klitynamy bulo vykorystano oryginal'nu model' kul'tyvuvannja kistkovomozkovyh klityn intaktnyh myshej linii' Balb/c u cherevnij porozhnyni oprominenoi' osobyny u gelevyh dyfuzijnyh kamerah, shho zabezpechuvaly dostup zhyvyl'nyh rechovyn ta sygnal'nyh molekul zzovni. Rezul'taty. Vyjavleno, shho klityny kistkovogo mozku myshej, jaki ne buly opromineni, piddavalysja bezposeredn'omu vplyvu faktoriv mikrootochennja organizmu oprominenoi' tvaryny-recypijenta. Tak, pry kul'tyvuvanni klityn v organizmi neoprominenyh myshej bulo vyjavleno nyz'ki pokaznyky kolonijeutvorennja u zv’jazku z vidsutnistju dodatkovogo vykydu rostovyh faktoriv. U toj zhe chas oprominennja tvaryn-recypijentiv kamer ta, menshoju miroju, obrobka i'h cyklofosfamidom, jakyj je cytostatykom, pidvyshhuvaly proliferatyvnu aktyvnist' kistkovomozkovyh klityn, jaki znahodylysja u kameri, shho svidchylo pro znachnyj vplyv faktoriv, jaki buly vydileni klitynamy u vidpovid' na diju ionizujuchoi' radiacii'. Vplyv sygnal'nyh molekul zdijsnjuvavsja kriz' stinku dyfuzijnoi' kamery, vyklykajuchy stymuljaciju kolonijeutvorjujuchoi' aktyvnosti. Pry c'omu slid zaznachyty, shho dlja peredachi sygnalu bezposerednij kontakt mizh klitynamy ne buv neobhidnym. Vysnovky. Stymuljujuchyj vplyv na kolonijeutvorjujuchu zdatnist' neoprominenyh gemopoetychnyh klityn-poperednykiv, pomishhenyh u dyfuzijni kamery, jakyj zdijsnjujet'sja klitynnymy ta pozaklitynnymy strukturamy, shho formujut' zovni mikrootochennja, pov'jazujet'sja iz dijeju rostovyh faktoriv, jaki vyrobljajut'sja oprominenymy klitynamy ta vplyvajut' na neopromineni klityny cherez stinku kamer, shho nedostupna dlja klityn, prote vil'no propuskaje dyfundujuchi molekuly. MATERIALS AND METHODS: To study the characteristics of interaction between the cells we implemented an original cultivation model with bone-marrow cells of intact Balb/c mice inserted in the diffusion chambers in peritoneum of irradiated animals (it provided the access of nutrients and signaling molecules from outside). RESULTS: As a result of investigations performed, we have determined the direct influence of microenvironment factors of irradiated mice organism on the non-irradiated bone-marrow cells. Thus, cultivation of the cells in the organism of non-irradiated mice revealed a low colony-forming activity due to the absence of additional growth factors release. At the same time an irradiation of the recipient mice and, to a lesser extent, a treatment by cytostatic agent cyclophosphamide increased the proliferative activity of bone-marrow cells in chambers, which indicated the pronounced impact of factors released by the cells as an effect of ionizing radiation. Influence of signaling molecules was realized through the wall of diffusion chamber, causing the stimulation of colony-forming activity. Moreover, we should mention that a direct contact between cells was not necessary for the delivery of a signal. CONCLUSIONS: Stimulating influence on the colony-forming activity of non-irradiated hematopoietic progenitor cells in diffusion chambers, which is performed by cellular and extra-cellular structures forming external microenvironment, is associated with the action of growth factors produced by irradiated cells and affecting non-irradiated cells through the chamber wall, which is impermeable for the cells but freely transmits diffusible molecules.