Expression of Collagen Gene in Different Passages of Human Fibroblasts Cultured in Vitro and Reactivity of Fibroblasts to EGF

Objective To investigate the expression of collagen ge ne in different passages of human fibroblasts cultured in vitro and the reactivity of fibroblasts to epithelial growth factor (EGF). Method Fib roblasts from human normal skin were taken for successive cell culture. RT-PCR and 3H-TdR were performed. Result The expressions o f collagen Ⅰ and Ⅲ mRNAs, and content of 3H-TdR decreased gradually as t he passages of cultured human fibroblasts increased. EGF promotes the proliferat ion of P 10 cultured fibroblast instead of the P 60 cultured fibroblas t. Conclusion The present results might provide partial exper imental data for establishing human fibroblast senescent model in vitro.