P056 Amelioration of indomethacin-induced gastric injury by hydroxy-omeprazole

Introduction Stomach ulcer is an illness that affects a considerable number of people worldwide. We investigated the effect of hydroxy-omeprazole on indomethacin-induced gastric injury in mice. Methods Gastric injury was induced by oral injection of indomethacin. Hydroxy-omeprazole (3 mg/kg) was given to B6 mice once daily for up to 7 days; the first dose was given 6 h after indomethacin administration. The mice were sacrificed on the fourth and seventh days, 4 h after administering the last dose of the samples. Results Total immunoglobulin G and A levels were increased in sera and fecal extracts of mice administrated with indomethacin and hydroxy-omeprazole compared with mice administrated with indomethacin alone.In histopathological analysis, immune cell infiltration on indomethacin-induced ulcerated gastric tissues was prevented by administration of hydroxy-omeprazole.In addition, hydroxy-omeprazole suppressed gene expression of inflammatory cytokines including COX, IL-1band IL-6 in indomethacin-induced ulcerated gastric tissues. Conclusion These results suggest that hydroxy-omeprazole ameliorated gastric injury by inhibiting expression of inflammatory cytokines.