Aging and radiation induced alterations of histones in regenerating rat liver.

Abstract We studied the age-related and radiation-induced changes in histones in regenerating liver of rats between 1 and 28 months of age. The examinations were carried out 30 h after a two-third hepatectomy, i.e. after the first wave of DNA and histone synthesis. Durng induced regeneration age-related changes in liver manifested themselves in decreasing the cellularity per gram wet weight, in slowing down the increase in DNA and histone contents per organ, in changing the mutual proportions of histone fractions H1, H2A + H2B, H4 and in increasing the H1° variant within histone H1. Radiation-induced latent injury (a dose of 5.7 Gy gamma radiation 30 min before partial hepatectomy) was manifested during induced regeneration in similar but much more profound changes with their earlier onset. Since the changes in regenerating liver were found not to be milder than in intact liver it means, that induced proliferation did not lead to elimination of altered cells or to their rejuvenation.