Expression of aquaporin 4,7,8 mRNA in neonatal rat model of necrotizing enterocolitis

Objective:To explore the expression changes of AQP4,7,8 mRNA in neonatal rat model of necrotizing enterocolitis (NEC) and discuss the clinical significance.Methods:20 neonatal rats were randomly divided into experimental group (n=10) and control group (n=10).NEC model of experimental group was established as the following method,one-day old Sprague-dawley (SD) rats were put into 99.99% carbon dioxide for 5 min and then 99% oxygen for 5 min before returned to their mothers.This was done once a day for 3 days.On the 4th day,all rats were sacrificed and the intestinal tissue located at the boundary of the ileum and cecum was obtained for quantification PCR.The data was calculated by the 2-△△Ct method following the software of REST-2005 to get the ratio (R).R1 meant AQP mRNA was down-regulated in the experimental group,R1 was opposite.Results:Compared with the control group,the ratio of AQP4,7,8 gene was less than one [(0.117± 0.129),(0.140±0.156),(0.134±0.140),R1 (P0.05)].Thereby,the expression of AQP4,7,8 mRNA was significantly different.Conclusion:Intestinal injury in NEC rat model is associated with a down-regulation of AQP4,7,8 mRNA expression,perhaps there is a reduction and redistribution in the expression of AQP4,7,8 gene.