Biodegradation of Di-n-butyl Phthalate by Pseudomonas aeruginosa

A new small capacity-wide extraction method was proposed for detection of its biodegradation in water.Results showed that the half-life(t_ 1/2)of di-n-butyl phthalate(DBP)biodegradation was 3.60 day when the concentration of DBP was 400 mg/L and the biomass concentration was 2 g/L.The biodegradation process conformed to the first-order kinetic model.Moreover,the whole degradation process could be divided into several steps:adsorption,desorption and degradation.Two metabolites of DBP degradation were identified as mono-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry,which confirmed the dioxygenate process during the hydrolysis of DBP.