Strain differences related to Ebola virus reproduction in peritoneal macrophages and in aorta explants of guinea pigs

: Reproduction of the Ebola strains (ES) virus causing lethality in guinea pigs as well as in peritoneal macrophages and aorta explants of animals was investigated in vitro and in vivo; besides, production of interferon-gamma (IFN-gamma) and of tumor necrosis factor-alpha (TNF-alpha) by macrophages and endotheliocytes of guinea pigs was also studied. The interplay "macrophage--ES" by the example of 2 models of susceptibility to ES demonstrates that the ES lethality is not unambiguously related only with a level of virus reproduction in macrophages. The interplay "endotheliocyte--ES" is indicative of that the ES lethality is inversely dependent on a level of production of the IFN-gamma and of TNF-alpha by endotheliocytes. In general, the Eboly fever lethality is not conditioned only by the ability or inability of ES to reproduce in macrophages and endotheliocytes; it also depends on a variety of pathogenetic factors, one of which could be the cytotoxic action of immune complexes shaping in the process of infection progression.