Additional file 2: Figure S2. of IMPACT is a GCN2 inhibitor that limits lifespan in Caenorhabditis elegans

Impt-1 expression in impt-1 +/– mutants and worms exposed to impt-1 RNAi. (a,b) Impt-1 mRNA expression in day 0 N2 (n = 3 pools of 150 worms) and impt-1 +/– (ok3233) (n = 3 pools of 150 worms) worms (a) or N2 worms treated with L4440 (control) (n = 3 pools of 150 worms) or impt-1 RNAi (n = 3 pools of 150 worms) from eggs (b). Data are presented as mean ± SEM and compared using unpaired Student t test, **P = 0.0097 in comparison to N2, *P = 0.036 in comparison to L4440. The experiments were performed once in triplicate. Raw data in Additional file 11: Table S2. (PDF 296 kb)