Electrochemical determination of human hemoglobin by using ferrocene carboxylic acid modified carbon powder microelectrode
2003
Abstract A mediator modified carbon powder microelectrode was developed and applied for the electrochemical determination of human hemoglobin in whole blood. The electrode body contained acetylene carbon black blended with ferrocene carboxylic acid. A quantitative oxidation of oxyhemoglobin to methemoglobin by the modified electrode was justified spectroscopically. The electrode responded to the addition of Hb within 10 s with the sensitivity of 64 nA g−1 dL in the linear range between 0.05 and 0.6 g dL−1. Thus, a sample solution of 1/50 allows for measurement of hemoglobin in human whole blood in the physiological important concentration range.
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