A development and an improvement of selectable markers in Pleurotus ostreatus transformation

Abstract Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3 ± 0.6 transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7 ± 11.5 transformants/μg plasmid DNA.