Protein profiling and molecular expression of Indian climbing perch on exposure to aquatic toxicants of Buckingham canal, Chennai, Tamil Nadu, India
2018
Fish provide excellent material for the study of mutagenic or carcinogenic potential of water samples, since they can metabolize, concentrate and store water-borne pollutants. Biomarkers are able to detect oxidative stress and DNA fragmentation which constitute early warning indicators assessable prior to severe alteration in single organism and the community as a whole. Comet assay has critically important applications in the field of toxicology ranging from aging and clinical investigations to genetic toxicology and molecular epidemiology. In the present study, the Indian climbing perch, Anabas testudineus from Buckingham canal, Chennai, Tami Nadu, India was selected for the investigation. In the six tissues (brain, gill, intestine, kidney, liver and muscle) of Anabas testudineus, protein bands ranging from 17 to 250kDa were observed and variable numbers of proteins were visible in each tissue. DNA damage demonstrated that there was a significant difference (p
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