Chemical Synthesis of Mono‐ and Bis‐Labeled Pre‐MicroRNAs

and other related fields. Most applicationsinvolve relatively short DNAs and RNAs; however thedemand for labeled long RNA (>60 nt) has increased inline with our growing appreciation of a rich, only partiallyunderstood biology of structured non-coding RNAs.A common secondary structure motif in RNA is thehairpin, which is composed of a terminal loop region anda double-stranded RNA stem that often bears mismatchesandbulges.Thismotifiscentraltoseveralbiologicalfunctionsincluding RNA maturation, RNA folding, ribozyme function,and protection of RNA from degradation.