Influence of low-level laser radiation on erythrocyte membranes

The purpose of this research was to investigate the influence of low-level laser radiation on erythrocyte membranes. The method of seif-hemolysis test of erythrocyte was employed in the research. Blood is taken from the vein of a healthy human. The blood was diluted by physiological salt water. Samples ofthe blood are divided into laser groups and control groups. Each sample of the blood was of 2m1 in volume and was put in a test tube. Each test tube of laser groups was radiated by He-Ne laser for about 40 minutes. The output power of laser vary from 1 mW to 8mW for different test tubes. After the laser radiation, the sample of the blood in test tubes was kept in 37CC for 24 hours, and then was tested for the haemolytic ratio. The result of the research showed that the haemolytic ratio of erythrocyte of laser groups was slightly larger than that of the control groups, and the bigger of the laser power, the larger of the haemolytic ratio of erythrocyte. This result indicates that the low-level laser radiation on blood can influence the membranes of red blood cell. This influence can be a stimulation to red blood cells. It can improve the membranous property and functions of red blood cell under some conditions. This result would be helpful to understand the mechanisms of the Intravascular Low-Level Laser Irradiation Therapy (ILLLIT).