Laboratory Diagnosis of HIV/AIDS Patients Complicated with Pneumocystis jirovecii Poneumonia

Pneumocystis jirovecii was detected in sputum samples and bronchoalveolar lavage fluid(BALF) ob-tained from HIV/AIDS patients complicated with Pneumocystis jirovecii poneumonia by Giemsa staining.CD4+ T lymphocytes of 500 patients were counted by flow cytometer.P.jirovecii positive rate in sputum samples(46.8%,845/1 806) was significantly lower than that of BALF(55.8%,106/190)(P0.05).The proportion of patients developing clinical symptoms in P.jirovecii positive cases(96.6%,816/845) was higher than that of P.jirovecii negative cases(64.0%,615/961)(P0.05).P.jirovecii positive rate increased with the decrease of CD4+ T lymphocyte number.P.jirovecii positive rates in cases with CD4+ 200×106/L,CD4+ 200×106/L-100×106/L,and CD4+ 100×106/L were 12.0%(6/50),39.0%(39/100),and 54.6%(191/350),respectively(P0.05).Giemsa staining is an efficient,simple and feasible method for P.jirovecii detection,relying on the experience and skill of the operator.