Bacterial leaf spot on Convolvulus arvensis caused by Pseudomonas sp.

Field bindweed (Convolvulus arvensis) is considered as one of the most destructive weeds in the fields and gardens worldwide. In summer 2017, during the investigation carried out in Mazandaran province, a significant number of field bindweed indicated the leaf spot symptoms. Hence, the samples were collected and brought to the phytobacteriology lab for further examination. Then, isolation, purification and biochemical characterization of fifteen isolates were performed based on the standard bacteriological methods. The pathogenicity test of selected isolates on field bindweed plant were evaluated as positive. In order to validate the results, partial of 16S rRNA region were sequenced and phylogenetic trees constructed as well. According to the phenotypic and molecular characteristics, field bindweed isolates were identified as Pseudomonas sp. To the best of the authors’ knowledge, the present study is the first report of bacterial leaf spot on Convolvulus arvensis caused by Pseudomonas sp. in Iran.