Accumulation and export of glycerol in unicellular green alga Chlamydomonas reinhardtii

Under hyperosmotic stress gametes as well as vegetative cells of Chlamydomonas reinhardtii accumulate/export osmolyte glycerol. The kinetics of accumulation/secretion of glycerol in gametes is similar to vegetative cells and differs only at osmolyte levels. At simultaneous action of osmotic stress and hypothermia, accumulation and secretion of glycerol are lower than those under osmotic stress at room temperature. Glycerol is not involved in adaptation of microorganism to hyper- and hypo-temperature stresses.