Relationship analysis of upland rice under shading condition based on RAPD

Sulistyowati Y, Lestari AP, Mulyaningsih ES. 2018. Relationship analysis of upland rice under shading condition based onRAPD Pros Sem Nas Masy Biodiv Indon 4: 190-194. Upland rice is tolerant crops on dry land and can be planted as a crop stream to thearea under the stands. However, the shade area under the stands often affect the growth of rice in the fields. Efforts to develop uplandrice varieties tolerant shade is necessary. Molekuler markers can be used to analysis genetic variability, inform cultivar identity andevolution study. RAPD (Randomly amplified polymorphic DNA) is one of the methods to analysis genetic variability. In a previousstudy, screening level of 200 rice genotypes had been done in a controlled shade using paranet.19 genotypes with high yield and 11genotypes with low yield under controlled shade were used in this study. The objective of this study was to assess the genetic variabilityof 30 rice genotypes that planted under the shade area using molekuler marker analysis. Thirty rice genotypes were analized using 20RAPD primer. Twelve out of 20 primer gave the polimorfic bands and then analyzed using the NTSYS 2.02 program. The resultsshowed a total of 72 RAPD loci, with an average of 6 fragmen per primer. The dendrogram for pooled data showed four clusters insimilarity coefficient 0.76. The number of genotypes of each cluster were 16, 9, 3 and 2 respectively (cluster 1,2,3,and 4). Theinformation of genetic variability is useful in the choice of parents for plant breeding and assess the variability of germplas.