THE INTERACTION OF BARLEY-α AMYLASE WITH ANTIOXIDANT FLAVONOID IN ANIONIC SURFACTANT SLS

The results of the studies of fluorescence quenching of Barley-α Amylase (B) by an antioxidant flavonoid Amantadine (AMT) in anionic surfactant Sodium Lauryl Sulphate (SLS) solution has been presented in this paper. The interaction of the anti-oxidant flavonoid with B was studied by UV/visible and fluorescence spectroscopic techniques. Using fluorescence study, the binding constants K for the binary complexes of antioxidant flavonoid and Barley-α Amylase were calculated. The number of binding sites was calculated. Using the calculated binding constants, thermodynamic parameters and the nature of the binding forces were determined. The calculated micelle parameters were also tabulated.