Expression of major histocompatibility complex-II on mouse cryptosporidiosis

To observe the treating function of Fructus Psoraleae and dihydroartemisinin on mouse infected with Cryptosporidium parvum and to research the regulating function of major histocompatibility complex-Ⅱ,60 mice were randomly divided into five groups: normal group A;positive control group-Ⅰ B;one-week treatment group C;positive control group-Ⅱ D;two-week treatment group E.Mice in group B,C,D,and E were injected subcutaneously with dexamethasone.When the mice were infected with C.parvum,they were treated with the medicine respectively.The intestine tissue was observed via HE.The expression of MHC-Ⅱ were observed by immunohistochemistry.The damaged intestine got improved and repaired,and a significant decrease of the amount of C.parvum oocysts in feces was shown in the treated groups(C,E) compared with positive control groups(B,D)(P0.05).In 1-week treatment group,the MHC-Ⅱ expression of group B was higher than that of normal group A.There was no difference between the expression of MHC-Ⅱ in group B and that in group C;in 2-week treatment group,there was no difference between the expression of MHC-Ⅱ in group E and that in group A.The MHC-Ⅱ expression of group D was lower than that of group A and B(P0.05).It's suggested that fructus Psoraleae and dihydroartemisinin are effective to treat mouse cryptosporidiosis.The expression of MHC-Ⅱ might be regulated by the mixture.