Gold immunochromatographic method for rapid detection of H3 and H7 subtypes avian influenza viruses.

Au-collosol was prepared by microwave,purified the monoclonal antibody of H3 and H7 subtypes AIV(H3-1D6 and H7-1F7),and optimized the combine condition,then,sprayed on the fiberglass,and obtained Au-marked pad.Coated the purified horse-anti-H3 and H7 polyclonal antibody on nitrocellulose membrane as the detection line,and goat-anti-Mus antibody as the quality control line.Detected the standard antigen of H3 and H7,expressed protein in Pichia pastoris,and the known sample,the result showed conformity with the hemagglutination test,hemagglutination inhibition assay,AC-ELISA and RT-PCR.Detected the standard antigen of H5 and H9,negative samples,IBV,NDV simultaneously,the result showed negative.This method has a simple operation,which could assess the result within 10 minutes,and the sensitivity was the same as hemagglutination test and hemagglutination inhibition assay.