Femtomolar Zn(II) affinity of minimal zinc hook peptides – a promising small tag for protein engineering
2013
The minimal zinc hook peptide of Rad50 and its alanine mutants form highly stable Zn(II) complexes. These peptides were successfully used as a small, efficient tag for reversible Zn(II)-mediated protein homodimerization. The high stability, its biological consequences and potential applications in protein engineering are discussed.
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