Two-photon fluorescence correlation microscopy for biophysical studies

Two-photon correlation spectroscopy enables the deeper insight into the living tissue in comparison to single photon spectroscopy and visualize the biophysical processes occurring there. We present the basic features of an experimental set up based on a research Nikon microscopy and preliminary experimental results of suitable dye with two- photon excitation for pharmacokinetic studies. Despite some technical problems the proposed design differs by its simplicity, requires relatively low-cost optics and has a reasonably low dispersion on the optical elements.