Determination of the water-soluble total sugars in Malussieboldii

Objective To determine the water-soluble total sugars in water extract from Malussieboldii.Methods The total sugars in water extract of Malussieboldii after acid hydrolysis and extracted with ethyl acetate were determined with the anthrone-sulfuric colorimetry in which the glucose was used as standard reference.Results The concentration of glucose showed a good linearity with absorbance in the range of 7～35 μg/mL at 620 nm(r=0.998 1,n=6).The average water soluble total sugars of three shipments of Malussieboldii was 13.1%,the average recovery was 94.1%,and RSD was 1.0 %(n=6).Conclusion The method can improve the absorption peak remarkably with differential spectrophotometry(△A method) to eliminate the colored matrix and organic interference,which can provide a reliable and repeatable method for determination of the water-soluble total sugars in Malussieboldii extractions.