Application of hollow fiber cooperative liquid phase microextraction in analysis of four hyoscyamines

The preferred conformations of anisodamine hydrobromide,atropine sulphate,scopolamine hydrobromide,scopolamine butylbromide were researched by cooperative liquid phase microextraction (CLPME) initially.In addition,the selective extraction and cooperative extraction mechanism of organic solvent to the standards were interpreted.The effect of charge transfer supermolecule formed by hollow fiber and hyoscyamines on the extraction efficiency was researched in the extraction procedure,and the electrophilic capability of N atom in formation of the four compounds was also studied.The contents of the four hyoscyamines in preparations were determined by liquid phase microextraction (LPME) combined with high performance liquid chromatography (HPLC).The calibration curves of the control articles were obtained in the concentration ranges of 0.05～5 mg/L (r0.99),RSD was lower than 7%,the average recoveries in raceanisodamine tablets and belladonna tablets were in the ranges of 95.0%～119.0% and 93.0%～95.0%,respectively.The detection limits of anisodamine hydrobromide,scopolamine butylbromide,atropine sulphate and scopolamine hydrobromide were 0.03,0.03,0.01 mg/L and 0.01 mg/L,respectively.