Interleukin 10 inhibits cytokine production of human AML cells
1996
Background : Unlike normal hematopoietic cells leukemic blasts from patients with AML constitutively express cytokines like IL1, GM-CSF and TNFα and it has been suggested that these cytokines may regulate growth and differentiation of the malignant cells. IL10 inhibits cytokine production of activated macrophages and T-helper 1 cells. We analysed whether IL10 can also suppress cytokine production and may inhibit growth of AML cells. Materials and methods : AML blasts of 18 patients were purified by immunomagnetic separation and cultured in serum-free medium in the presence of cytokines. The production of cytokines was analysed by ELISA, DNA synthesis by 3 H-thymidine incorporation and mRNA expression of cytokine genes by semiquantitative RT-PCR. Results : Our results confirm previous results that AML blasts produce a variety of cytokines such as GM-CSF, IL1α, IL1β, IL6 and TNFα. AML cells were induced to proliferation by G-CSF, GM-CSF, IL3, IL1β and SCF to a different extent. In contrast, IL10 significantly inhibited the cytokine production at the mRNA and protein level and spontaneous thymidine uptake in a dose-dependent way. This inhibition could be abrogated by IL10 specific antibodies. Conclusion : These observations suggest an inhibitory effect of IL10 on the proliferation of cultured AML blasts most likely through suppression of endogenous cytokines.
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