Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid-liquid microextraction followed by HPLC-FLD

Abstract The discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 26-1 factorial design was used to identify the significant variables (p 0.9999), repeatability ( 91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63 - 7.44 ng mL-1) and trans-RSV (169 - 2616 ng mL-1) in different wine varieties. Comparison with literature point the overall advantages of the new method.